APPLICATION AND ADAPTATION OF DOT IMMUNOASSAY TO DETECT AND MEASURE RUBELLA VIRUS ANTIBODY
نویسندگان
چکیده مقاله:
A dot immunobinding assay ( DIA) was used for a quantitative and qualitative assay of rubella antibody. Purified antigen and conjugated anti-human immunoglobulin (RAHlg) were prepared. Nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. The reacting antibodies were visualized by a peroxidase system. Development of a colored insoluble substrate was taken as a positive result. The adapted DIA was applied to test 105 serum samples. The sensitivity and specificity and immune titer of DIA were compared with hemagglutination inhibition (HI) test.
منابع مشابه
application and adaptation of dot immunoassay to detect and measure rubella virus antibody
a dot immunobinding assay ( dia) was used for a quantitative and qualitative assay of rubella antibody. purified antigen and conjugated anti-human immunoglobulin (rahlg) were prepared. nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. the reacting antibodies were visualized by a peroxidase system. development of a colored insoluble substrate was t...
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عنوان ژورنال
دوره 15 شماره 3
صفحات 171- 174
تاریخ انتشار 2001-11
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